The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Ĭompeting interests: The authors have declared that no competing interests exist. 31600515, ) and the Natural Science Foundation of Hunan Province, China (Grant No. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the paper and its Supporting Information files.įunding: This work was supported by the National Science Foundation for Young Scientists of China (Grant No. Received: AugAccepted: ApPublished: April 27, 2020Ĭopyright: © 2020 Xu et al. Morais, Universidade de Coimbra, PORTUGAL (2020) Isolation and characterization of Bacillus subtilis strain 1-L-29, an endophytic bacteria from Camellia oleifera with antimicrobial activity and efficient plant-root colonization. fructicola as well as the root vascular tissues of Arabidopsis thaliana.Ĭitation: Xu J-X, Li Z-Y, Lv X, Yan H, Zhou G-Y, Cao L-X, et al. Fluorescence microscopic analysis showed that strain 1-L-29 gfp r thoroughly colonized the root surfaces of C. This strain remained at a stable concentration in the root more than 20 d after inoculation. oleifera and successfully colonized root, stem, and leaf tissues. Strain 1-L-29 gfp r was re-introduced into C. This plasmid had good segregational stability. To facilitate further microecological studies of this strain, a rifampicin-resistant, green fluorescent protein (GFP)-labeled strain, 1-L-29 gfp r, was created using protoplast transformation. In addition, this strain produced indole acetic acid, solubilized phosphate, grew on N-free media, and produced siderophores. asianum, Fusarium proliferatum, Agaricodochium camellia, and Pseudomonas syringae. Strain 1-L-29 demonstrated antagonistic activity against C. subtilis strain 1-L-29 being the most efficient. The antagonistic effects of these strains on C. Bacterial cultures were identified based on analyses of 16S rDNA sequences most strains belonged to the genus Bacillus.
oleifera anthracnose, 41 endophytes were isolated from the stems, leaves, and roots of C. To find a suitable biocontrol agent for C. oleifera anthracnose, which is caused by Colletetrichum fructicola. oleifera is strongly negatively affected by C. Camellia oleifera, an oil-producing plant, is widely grown in warm, subtropical, hilly regions in China. Endophytic bacteria, which are common in plant tissues, may help to control plant pathogens and enhance plant growth.
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